Reproduction+(heredity)

Jess Mertz Marilin Ocelotl

Hypothesis: If overtime more light passes through the unknown test tubes, reproduction is happening which means the unknown is living.

Materials: 1. Unknown substances (a,b &c) 2. 4 test tubes 3. Sterile LB Broth 4. Spectrophotometer with cuvettes 5. 10ml pipette and pump 6. Glass marking pencil 7. Sterile swab 8. Test tube rack 9. marking pencil

Procedure: 1. Label the four test tubes as known, unknown A, unknown B, and unknown C. 2. Add 10 ml of Sterile LB broth to each tube 3. obtain a swab and gently place it on a colony of the unknown substance B in the Petri dish 4. Remove swab from petri dish and place in test tube B and mix

5. obtain a swab and gently place it on a colony of the unknown substance C in the Petri dish 6. Remove swab from petri dish and place in test tube C and mix 7. obtain a swab and gently place it on a colony of the unknown substance D in the Petri dish 8. Remove swab from petri dish and place in test tube D and mix 9. Take initial spectrophotometer readings from Tubes A, B, C and D. 10. Create a table to record spectrophotometer for all tests (you will need to record spectrophotometer readings for four days ) 11. Repeat spectrophotometer readings for four days and record in the previous created table. 12. Graph data recorded in table.

Control: In this experiment the control is the known test tube.

Data:
 * Days || Known || Unknown A || Unknown B || Unknown C ||
 * Day 1 || 99.8 || 95.6 || 36.8 || 99.8 ||
 * Day 2 || 121.6 || 89.4 || 31.4 || 105.4 ||
 * Day 3 || 101.6 || 78.6 || 32.2 || 43.0 ||
 * Day 4 || 30.8 || 37.6 || 14.2 || 24.4 ||