Hypothesis:
If overtime more light passes through the unknown test tubes, reproduction is happening which means the unknown is living.
Materials:
1. Unknown substances (a,b &c)
2. 4 test tubes
3. Sterile LB Broth
4. Spectrophotometer with cuvettes
5. 10ml pipette and pump
6. Glass marking pencil
7. Sterile swab
8. Test tube rack
9. marking pencil
Procedure:
1. Label the four test tubes as known, unknown A, unknown B, and unknown C.
2. Add 10 ml of Sterile LB broth to each tube
3. obtain a swab and gently place it on a colony of the unknown substance B in the Petri dish
4. Remove swab from petri dish and place in test tube B and mix
5. obtain a swab and gently place it on a colony of the unknown substance C in the Petri dish 6. Remove swab from petri dish and place in test tube C and mix 7. obtain a swab and gently place it on a colony of the unknown substance D in the Petri dish 8. Remove swab from petri dish and place in test tube D and mix 9. Take initial spectrophotometer readings from Tubes A, B, C and D. 10. Create a table to record spectrophotometer for all tests (you will need to record spectrophotometer readings for four days ) 11. Repeat spectrophotometer readings for four days and record in the previous created table. 12. Graph data recorded in table.
Control: In this experiment the control is the known test tube.
Marilin Ocelotl
Hypothesis:
If overtime more light passes through the unknown test tubes, reproduction is happening which means the unknown is living.
Materials:
1. Unknown substances (a,b &c)
2. 4 test tubes
3. Sterile LB Broth
4. Spectrophotometer with cuvettes
5. 10ml pipette and pump
6. Glass marking pencil
7. Sterile swab
8. Test tube rack
9. marking pencil
Procedure:
1. Label the four test tubes as known, unknown A, unknown B, and unknown C.
2. Add 10 ml of Sterile LB broth to each tube
3. obtain a swab and gently place it on a colony of the unknown substance B in the Petri dish
4. Remove swab from petri dish and place in test tube B and mix
5. obtain a swab and gently place it on a colony of the unknown substance C in the Petri dish
6. Remove swab from petri dish and place in test tube C and mix
7. obtain a swab and gently place it on a colony of the unknown substance D in the Petri dish
8. Remove swab from petri dish and place in test tube D and mix
9. Take initial spectrophotometer readings from Tubes A, B, C and D.
10. Create a table to record spectrophotometer for all tests (you will need to record spectrophotometer readings for four days )
11. Repeat spectrophotometer readings for four days and record in the previous created table.
12. Graph data recorded in table.
Control: In this experiment the control is the known test tube.
Data: