- Materials
4 test tubes
unknown substances
warm water
sugar
4 balloons
glucose stick
-Procedure
Step 1:
1.) Test Tube A- fill with unknown A with 1.25g, 2/3 of the TT with water. and 4.3g of sugar
2.) Test Tube B- fill with unknown B with 1.25g,2/3 of the TT with water, and 4.3g of sugar
3.) Test Tube C- fill with unknown C with 1.25g ,2/3 of the TT with water, and 4.3g of sugar
4.) Test Tube D- fill with 2/3 of the TT with water and 4.3g of sugar
Step 2:
For each test tube:
1.)Place one glucose strip in mixtures
2.) Place one balloon on each test tube.
3.) Record data after 30 minutes record balloon inflation and bubbles
4.) After 24 hours record Bromo , balloon inflation, bubbles and glucose:
5.) To test for CO2: Bromothymol Blue in a separate test tube
6.) Take balloon off original test tube (keep air in balloon)
7.) Release air into Bromothymol Blue test tube.
8.) Record results
To test for glucose:
Place one glucose strip in mixtures
Hypothesis:
- If the balloons inflate on the unknown test tubes CO2 is present and if the glucose strip stays the same color, then the glucose was used for energy.
Observations
Initial:
-test tube A: most of it is mixed together, rest of it sits at the bottom- color changed to yellow- ballon not inflated- few froth bubbles
-test tube B: everything mixed together, sandy color, balloon somewhat inflated- a lot of froth bubbles- substance starting to rise
-test tube C : substances sinks to the bottom- balloon not inflated- no froth bubbles- clear
-test tube D: sugar disintegrated into water- balloon not inflated- no froth bubbles- clear
INITIAL PICTURES:
AFTER 30 MINUTES:
Observations:
After 30 minutes:
-test tube A: more of it is mixed together- balloon still not inflated- some froth bubbles- still yellow
-test tube B: all mixed together- lots of froth bubbles- balloon inflated- still a sandy color
-test tube C: mixture still sitting at the bottom- clear- balloon not inflated- no froth bubbles
-test tube D: sugar mixed in with water- clear - no froth bubbles - balloon not inflated.
AFTER 24 HOURS:
Observations:
- test tube A: rest of mixture sitting at the bottom- balloon somewhat inflated- top clear bottom yellow- no froth bubbles
-test tube B: mixture is still completely mixed- bubbles are rising to top of mixture(Carbon dioxide)- balloon very inflated- still a sandy white color- no froth bubbles
-test tube C: all mixture sitting at bottom- sandy at bottom clear at top- balloon not inflated- no froth bubbles- looks like water.
-test tube D: sugar is mixed in with water- clear color- no froth bubbles- balloon not inflated.
GLUCOSE TEST:
-test tube A: did not use glucose to produce carbon dioxide.
-test tube B: used the glucose as energy to produce carbon dioxide
-test tube C: did not use glucose to produce carbon dioxide
BROMO BLUE:
test tube B had carbon dioxide present- it changed from blue to orange- the rest of the test tubes did not have carbon dioxide present.
- Materials
4 test tubes
unknown substances
warm water
sugar
4 balloons
glucose stick
-Procedure
Step 1:
1.) Test Tube A- fill with unknown A with 1.25g, 2/3 of the TT with water. and 4.3g of sugar
2.) Test Tube B- fill with unknown B with 1.25g,2/3 of the TT with water, and 4.3g of sugar
3.) Test Tube C- fill with unknown C with 1.25g ,2/3 of the TT with water, and 4.3g of sugar
4.) Test Tube D- fill with 2/3 of the TT with water and 4.3g of sugar
Step 2:
For each test tube:
1.)Place one glucose strip in mixtures
2.) Place one balloon on each test tube.
3.) Record data after 30 minutes record balloon inflation and bubbles
4.) After 24 hours record Bromo , balloon inflation, bubbles and glucose:
5.) To test for CO2: Bromothymol Blue in a separate test tube
6.) Take balloon off original test tube (keep air in balloon)
7.) Release air into Bromothymol Blue test tube.
8.) Record results
To test for glucose:
Place one glucose strip in mixtures
Hypothesis:
- If the balloons inflate on the unknown test tubes CO2 is present and if the glucose strip stays the same color, then the glucose was used for energy.
Observations
Initial:
-test tube A: most of it is mixed together, rest of it sits at the bottom- color changed to yellow- ballon not inflated- few froth bubbles
-test tube B: everything mixed together, sandy color, balloon somewhat inflated- a lot of froth bubbles- substance starting to rise
-test tube C : substances sinks to the bottom- balloon not inflated- no froth bubbles- clear
-test tube D: sugar disintegrated into water- balloon not inflated- no froth bubbles- clear
INITIAL PICTURES:
AFTER 30 MINUTES:
Observations:
After 30 minutes:
-test tube A: more of it is mixed together- balloon still not inflated- some froth bubbles- still yellow
-test tube B: all mixed together- lots of froth bubbles- balloon inflated- still a sandy color
-test tube C: mixture still sitting at the bottom- clear- balloon not inflated- no froth bubbles
-test tube D: sugar mixed in with water- clear - no froth bubbles - balloon not inflated.
AFTER 24 HOURS:
Observations:
- test tube A: rest of mixture sitting at the bottom- balloon somewhat inflated- top clear bottom yellow- no froth bubbles
-test tube B: mixture is still completely mixed- bubbles are rising to top of mixture(Carbon dioxide)- balloon very inflated- still a sandy white color- no froth bubbles
-test tube C: all mixture sitting at bottom- sandy at bottom clear at top- balloon not inflated- no froth bubbles- looks like water.
-test tube D: sugar is mixed in with water- clear color- no froth bubbles- balloon not inflated.
GLUCOSE TEST:
-test tube A: did not use glucose to produce carbon dioxide.
-test tube B: used the glucose as energy to produce carbon dioxide
-test tube C: did not use glucose to produce carbon dioxide
BROMO BLUE:
test tube B had carbon dioxide present- it changed from blue to orange- the rest of the test tubes did not have carbon dioxide present.